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Autoantibodies against desmoglein 1, desmoglein 3, envo¬plakin, BP180, BP230, collagen type VII

Dermatology Mosaic 7

Dermatology Mosaic 7: oesophagus and split skin (top and middle left), BP230 gC and BP180-NC16A-4X (top and middle right), desmoglein 1 and 3 (bottom left and right): antibodies against epidermal basement membrane

  • Screening and differentiation test for detection of skin-specific antibodies.
  • Indication: bullous autoimmune dermatoses.
  • Antibodies against prickle cell desmosomes react with surface antigens of keratinocytes. Tissue sections of oesophagus and tongue show a granular fluo¬rescence of the intercellular matter in the whole stratum spinosum, but differentiation between desmoglein 1 and desmoglein 3 is difficult. When specific transfected cells are employed in addition, a targeted diagnosis in a single test run is possible.
  • Antibodies against basal membrane structures react with salt-split skin. Anti-BP180, anti-BP230 and anti-LAD97 cause staining of the epidermal side, and antibodies against laminin 5, collagen type VII and other antigens staining of the dermal side of the salt-split skin.
  • When autoantibodies against BP180 or BP230 are present, the epidermal basement membrane in the oesophagus or tongue is visible as a fine linear colouring between the stratum basale and the connective tissue. These antibodies can be differentiated by means of BP180-NC16A-4X coated BIOCHIPs and cells specifically transfected with BP230 (globular C-terminal domain (gC)).

Technical info and Images credit to EUROIMMUN AG