Autoantibodies against endomysium
Liver and gliadin (GAF-3X) antigen
Primate liver and gliadin (GAF-3X) BIOCHIPs: antibodies against endomysium and gliadin
- Detection of antibodies against endomysium and gliadin.
- Indications: gluten-sensitive enteropathy (coeliac disease, non-tropical sprue), Duhring's herpetiform dermatitis.
- Autoantibodies against endomysium react with many types of tissue, e.g. primate oesophagus. The most suitable substrate, however, is primate liver: in the case of a positive sample, filamentous linings of the intralobular sinusoids react.
- With the gliadin (GAF-3X)-coated BIOCHIP, antibodies against gliadin can be analysed in one and the same test procedure.
- Both anti-endomysium antibodies and antibodies against gliadin (class IgA) are reliable serological markers for an active gluten-sensitive enteropathy. Therefore, their determination can in many cases replace endoscopy and biopsy.
Coeliac Disease Profile
The serological detection of disease-specific antibodies (IgA and IgG) is an essential element of coeliac disease diagnostics. Especially IgA antibodies against tTG are considered the most specific and sensitive indicator. A 10-fold increased antibody concentration of the upper threshold value of the normal range (10x upper limit of normal, > 10 x ULN) is considered as a threshold value for the diagnosis of coeliac disease without an additional biopsy. In addition, the gliadin-GAF-3X antigen was developed for the detection of IgA and IgG antibodies directed against relevant, deamidated gliadin epitopes.
- Parallel qualitative in vitro determination of human antibodies against tissue transglutaminase (tTG) and gliadin-analogue fusion peptide (GAF-3X)
- Identification of a possible IgA deficiency using serum/plasma control bands
Technical info and Images credit to EUROIMMUN AG