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Autoantibodies against endomysium

Liver and gliadin (GAF-3X) antigen

Primate liver and gliadin (GAF-3X) BIOCHIPs: antibodies against endomysium and gliadin

  • Detection of antibodies against endomysium and gliadin.
  • Indications: gluten-sensitive enteropathy (coeliac disease, non-tropical sprue), Duhring's herpetiform dermatitis.
  • Autoantibodies against endomysium react with many types of tissue, e.g. primate oesophagus. The most suitable substrate, however, is primate liver: in the case of a positive sample, filamentous linings of the intralobular sinusoids react.
  • With the gliadin (GAF-3X)-coated BIOCHIP, antibodies against gliadin can be analysed in one and the same test procedure.
  • Both anti-endomysium antibodies and antibodies against gliadin (class IgA) are reliable serological markers for an active gluten-sensitive enteropathy. Therefore, their determination can in many cases replace endoscopy and biopsy.

Coeliac Disease Profile

The serological detection of disease-specific antibodies (IgA and IgG) is an essential element of coeliac disease diagnostics. Especially IgA antibodies against tTG are considered the most specific and sensitive indicator. A 10-fold increased antibody concentration of the upper threshold value of the normal range (10x upper limit of normal, > 10 x ULN) is considered as a threshold value for the diagnosis of coeliac disease without an additional biopsy. In addition, the gliadin-GAF-3X antigen was developed for the detection of IgA and IgG antibodies directed against relevant, deamidated gliadin epitopes.

  • Parallel qualitative in vitro determination of human antibodies against tissue transglutaminase (tTG) and gliadin-analogue fusion peptide (GAF-3X)
  • Identification of a possible IgA deficiency using serum/plasma control bands

Technical info and Images credit to EUROIMMUN AG